发明名称 |
Annealing control primer and its uses |
摘要 |
The present invention relates to an annealing control primer for improving annealing specificity in nucleic acid amplification and its applications to all fields of nucleic acid amplification-involved technology. The present primer comprises (a) a 3′-end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5′-end portion having a pre-selected arbitrary nucleotide sequence; and (c) a regulator portion positioned between said 3′-end portion and said 5′-end portion comprising at least one universal base or non-discriminatory base analog, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperature. |
申请公布号 |
US8124346(B2) |
申请公布日期 |
2012.02.28 |
申请号 |
US20090458702 |
申请日期 |
2009.07.21 |
申请人 |
CHUN JONG-YOON;SEEGENE, INC. |
发明人 |
CHUN JONG-YOON |
分类号 |
C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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