INDUCED SPORULATION SCREENING METHOD

摘要

The present invention relates to a method of screening for an improved enzyme variant, said method comprising the steps of: (i) providing a recombinant host cell capable of sporulating comprising a polynucleotide encoding a sporulation factor and a polynucleotide encoding an enzyme variant which are operably linked to an inducible promoter; (ii) culturing the host cell under conditions suitable to induce the formation of spores; (iii) culturing the spores obtained in step (ii) in a medium containing a substrate for the enzyme variant; and (iv) determining the activity of the enzyme variant.