METHOD AND DEVICE FOR PRESERVING CELL NUCLEI

摘要

The invention relates to a method for cryopreserving biological material comprising biological cells (1) having cell membranes (2), cell nuclei (3), cytoplasm (4), and cell organelles (5), comprising the steps of preparing the biological cells (1) such that the cell membranes (2) are interrupted, dehydrating the cell nuclei (3), and setting preservation conditions under which the cell nuclei (3) display a cryopreserved state. In addition, the cell membranes (2), cytoplasm (4), cell organelles (5), and/or parts thereof can be subjected to the dehydration and cryopreservation. The invention further relates to the use of alcohols, proteins, sugars, electrolytes, polyanions, polycations, polymers, oils, or gels as cryoprotective agents, and to a device for cryopreserving cell nuclei (3) of biological cells (1).