发明名称 |
METHOD FOR PRODUCING A CELL CAPABLE OF HIGH-YIELD PRODUCTION OF HETEROPROTEINS |
摘要 |
The present invention provides a cell capable of high-yield production of polypeptides and a method for producing the same. The present invention relates to a method for producing a cell capable of high-yield production of a desired polypeptide, wherein a strongly bicarbonate transporter-expressing cell into which DNA encoding the desired polypeptide has been introduced is cultured in the presence of a high concentration of methotrexate and a cell capable of high-yield production of the desired polypeptide is selected from among surviving cells. |
申请公布号 |
US2012045795(A1) |
申请公布日期 |
2012.02.23 |
申请号 |
US201013138909 |
申请日期 |
2010.04.21 |
申请人 |
TABUCHI HISAHIRO;SUGIYAMA TOMOYA |
发明人 |
TABUCHI HISAHIRO;SUGIYAMA TOMOYA |
分类号 |
C12P21/06;C12N1/19;C12N1/21;C12N1/38;C12N5/02;C12N5/10;C12N15/74;C12N15/79;C12N15/82 |
主分类号 |
C12P21/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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