METHOD FOR INACTIVATING PROTEASES BY PH CHANGE IN A LIQUID OBTAINED FROM A CELL CULTURE
摘要
The invention relates to a method for inactivating proteases by multiple change of pH in the cell culture supernatant at the beginning of the operation of purifying biopharmaceuticals. First of all a pH of 3-5, and subsequently a pH of 7-9, is set.
申请公布号
WO2012022688(A1)
申请公布日期
2012.02.23
申请号
WO2011EP63915
申请日期
2011.08.12
申请人
BOEHRINGER INGELHEIM INTERNATIONAL GMBH;JACOBI, ALEXANDER;AMBROSIUS, DOROTHEE;DOBBERTHIEN, PHILINE;ECKERMANN, CHRISTIAN;NOTHELFER, FRANZ
发明人
JACOBI, ALEXANDER;AMBROSIUS, DOROTHEE;DOBBERTHIEN, PHILINE;ECKERMANN, CHRISTIAN;NOTHELFER, FRANZ
