发明名称 |
METHODS AND COMPOSITIONS FOR SEQUENCE-SPECIFIC PURIFICATION AND MULTIPLEX ANALYSIS OF NUCLEIC ACIDS |
摘要 |
Methods and materials for determining the presence of at least one nucleic acid in a sample are provided, said methods comprising (1) a purification step using sequence specific hybrid capture; (2) an amplification step; and (3) a detection step comprising contacting the target nucleic acid with a plurality of detectably labeled nucleic acid detection probes, wherein each (a) bears a different detectable label from the other detection probes, and/or (b) has a different melting temperature from probes bearing the same detectable label. Also disclosed are compositions and kits for use in such a method.
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申请公布号 |
US2012045756(A1) |
申请公布日期 |
2012.02.23 |
申请号 |
US201113110660 |
申请日期 |
2011.05.18 |
申请人 |
ROTHMANN THOMAS;NAZARENKO IRINA;O'NEIL DOMINIC;VIRMANI ARVIND;LOWE BRIAN;AGARWAL SHIULI;BASHAM HOLLY;QIAGEN GAITHERSBURG, INC. |
发明人 |
ROTHMANN THOMAS;NAZARENKO IRINA;O'NEIL DOMINIC;VIRMANI ARVIND;LOWE BRIAN;AGARWAL SHIULI;BASHAM HOLLY |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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