发明名称 |
Variant of KpnI restriction endonuclease |
摘要 |
Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.
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申请公布号 |
US8119382(B2) |
申请公布日期 |
2012.02.21 |
申请号 |
US20060065384 |
申请日期 |
2006.08.18 |
申请人 |
NAGARAJA VALAKUNJA;SARAVANAN MATHESHWARAN;ZHU ZHENYU;NEW ENGLAND BIOLABS, INC.;INDIAN INSTITUTE OF SCIENCE |
发明人 |
NAGARAJA VALAKUNJA;SARAVANAN MATHESHWARAN;ZHU ZHENYU |
分类号 |
C12N9/22 |
主分类号 |
C12N9/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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