发明名称 |
Nucleic acid amplification primers for PCR-based clonality studies of the TCR-beta gene |
摘要 |
<p>The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a V²-J² TCRB rearrangement, and optionally a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a V»-J» IGL rearrangement, a D²-J² TCRB rearrangement, a V³-J³ TCRG rearrangement, a V´-J´ TCRD rearrangement, a D´-D´ TCRD rearrangement, a D´-J´ TCRD rearrangement, a V´-D´ TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-ICrH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.</p> |
申请公布号 |
EP2418287(A2) |
申请公布日期 |
2012.02.15 |
申请号 |
EP20100185191 |
申请日期 |
2003.10.13 |
申请人 |
ERASMUS UNIVERSITEIT ROTTERDAM |
发明人 |
VAN DONGEN, JACOBUS JOHANNES MARIA;LANGERAK, ANTHONIE WILLEM;SCHUURING, EDUARDUS MARIA DOMINICUS;SAN MIQUEL, JESUS FERNANDO;GARCIA SANZ, RAMON;PARREIRA, ANTONIO;SMITH, JOHN LEWIS;LAVENDER, FRANCES LOUISE;MORGAN, GARETH JOHN;EVANS, PAUL ANTHONY STUART;KNEBA, MICHAEL;HUMMEL, MICHAEL;MACINTYRE, ELIZABETH ANNE;BASTARD, CHRISTIAN;DAVI, FREDERIC BERNARD LOUIS;BRUEGGEMANN, MONIKA |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|