Polymer-supported culture systems

摘要

Producing a degradable biomaterial, comprises: (a) providing at least a three-arm polyethylene glycol component; (b) providing at least one oligomeric or polymeric component functionalized with at least two acrylate-, vinyl sulfone- or maleimide groups, where the oligomeric or polymeric component comprises at least two-arm polyethylene glycol component, polyvinyl alcohol, polypropylene glycol, gelatin or fibrinogen; (c) cross-linking the components provided in the steps (a) and (b); and (d) obtaining the degradable biomaterial. Producing a degradable biomaterial, comprises: (a) providing at least a three-arm polyethylene glycol component, where at least three arms of the polyethylene glycol component are functionalized with one mercaptoalkanoic acid with 1-10C; (b) providing at least one oligomeric or polymeric component functionalized with at least two acrylate-, vinyl sulfone- or maleimide groups, where the oligomeric or polymeric component comprises at least two-arm polyethylene glycol component, polyvinyl alcohol, polypropylene glycol, gelatin or fibrinogen; (c) cross-linking the components provided in the steps (a) and (b); and (d) obtaining the degradable biomaterial. Independent claims are also included for (1) the degradable biomaterial, comprising at least three-arm polyethylene glycol component, which is crosslinked by a multi-functional connection element with at least one oligomeric or polymeric component, where the multifunctional connection element comprises (i) a first ester group, (ii) a thioether group with 1-10C, and (iii) a second ester group or an electron withdrawing group; and (2) a kit for producing the degradable biomaterial comprising (ai) at least three-arm polyethylene glycol component, where at least three arms of the polyethylene glycol component is functionalized with a respective mercaptoalkanoic acid with 1-10 carbon atoms, and (bi) at least one oligomeric or polymeric component functionalized with at least two acrylate, vinyl sulfone or maleimide group.