ANTIBODY FOR ASSAY OF ADAMTS13 ACTIVITY AND METHOD FOR ASSAYING THE ACTIVITY

摘要

It is intended to provide an antibody, especially monoclonal antibody, useful for the determination of ADAMTS13 activity, and provide a method of determining the activity of ADAMTS13. Further, it is intended to provide a monoclonal antibody that exhibits specific reactivity to any antigen determinant site occurring when ADAMTS13 is caused to act on a partial peptide of VWF able to become a substrate or VWF as a substrate but has no specific reactivity to complete VWF molecules, and to provide a usage of the antibody. These have been achieved by success in obtaining of a monoclonal antibody (anti-N-10 monoclonal antibody) having specific reactivity to cleavage site at which a partial peptide of VWF is specifically cut by ADAMTS13 and by finding of a method of determining the ADAMTS13 activity by the use of this monoclonal antibody.