MULTI-COLOR FLUORESCENCE ENHANCEMENT FROM A PHOTONIC CRYSTAL SURFACE

摘要

A photonic crystal substrate exhibiting resonant enhancement of multiple fluorophores has been demonstrated. The device, which can be fabricated uniformly from plastic materials over a ~3x5 in2 surface area by nanoreplica molding, features a 1 -D periodic grating structure; (16, 18) which utilizes two distinct resonant modes to enhance electric field stimulation of a. first dye excited by a first laser (31) (e.g., ?=632.8 nm laser exciting cyantne-5) and a second dye excited by a second laser (32) (e.g., ?=532 nm laser exciting cyanine-3). The first and second lasers could be replaced by a single variable wavelength (tunable) laser. Resonant coupling of the laser excitation to the photonic crystal surface is obtained for each wavelength at a distinct incident angle ?. The photonic crystal is capable of amplifying the output of any fluorescent dye with an excitation wavelength in a given wavelength range (e.g., the range 532 nm < ? < 660 nm) by selection of an appropriate incident angle. The device can be used for biological assays that utilize multiple fluorescent dyes within a single imaged area, such as gene expression microarrays.