Method for eliminating unwanted radiation portions from light detected from illuminated sample by laser scanning microscope, involves passing acousto optical tunable filter through two pole components

摘要

The method involves actuating an acousto optical tunable filter (AOTF). Illuminating light is faded out in a light case or a beam detour unit. A sample light is irradiated toward a detector. Unwanted light is faded out by the AOTF. The AOTF is passed through two pole components when irradiation takes place in two incident directions stretching along a plane, which is vertical to a propagation plane of a longitudinal wave of the AOTF and the irradiating directions symmetrical to the propagation direction. Independent claims are also included for the following: (1) a laser scanning microscope (2) a method for operating a laser scanning microscope.