System and method for a microfluidic calorimeter

摘要

Systems and methods are disclosed herein for a microfluidic calorimeter apparatus. A microfluidic calorimeter system includes a calorimetry apparatus and a processor in connection with the apparatus. The apparatus includes a microfluidic laminar flow channel connected to two inlets for flowing fluid into the laminar flow channel. Below the laminar flow channel is a plurality of microscale temperature sensors at known positions in the channel. The processor is in connection with the discrete temperature sensors and determines a calorimetry measurement based on local temperatures derived from data output by the microscale temperature sensors and the respective positions of the sensors in the channel.

主权项

1. A method for calorimetry comprising:
providing a calorimetry apparatus comprising:
a microfluidic laminar flow channel;two inlets in fluid connection with the microfluidic laminar flow channel, the inlets allowing fluid to flow into the microfluidic laminar flow channel; anda plurality of microscale temperature sensors disposed below the microfluidic laminar flow channel at known positions relative to boundaries of the microfluidic laminar flow channel; continuously flowing fluids from each of the two inlets into the microfluidic laminar flow channel such that a chemical reaction occurs at least at a diffusion interface of the fluids, wherein a ratio of fluid from a first one of the inlets to fluid from a second one of the inlets varies across the diffusion interface; receiving data output from each of the plurality of microscale temperature sensors; obtaining a local temperature at each of the respective positions of the plurality of microscale temperature sensors based on the data output from each of the plurality of microscale temperature sensors; processing the data output from each of the plurality of microscale temperature sensors to determine a temperature change at each of the known positions along the microfluidic laminar flow channel; calculating a first calorimetry measurement indicative of energetics associated with the chemical reaction at a first location where the ratio of the fluid from the first inlet to the fluid from the second inlet has a first value based on the temperature change at the respective position of at least a first one of the microscale temperature sensors in the microfluidic laminar flow channel; calculating a second calorimetry measurement indicative of energy associated with the chemical reaction at a second location, different from the first location, where the ratio of the fluid from the first inlet to the fluid from the second inlet has a second value, different from the first value, based on the temperature change at the respective position of at least a second one of the microscale temperature sensors in the microfluidic laminar flow channel.