| 摘要 |
<p>The present invention provides a liquid reflux reaction control device comprising:
a reaction vessel having one or a plurality of wells configured to accommodate a sample;
a heat exchange vessel provided in contact with the reaction vessel so as to conduct heat to the reaction vessel, and comprising an inlet and an outlet respectively for introducing and draining a liquid of a predetermined temperature;
a plurality of liquid reservoir tanks provided with a temperature-controllable heat source for maintaining liquids of predetermined temperatures;
a tubular flow channel that connects the inlet and the outlet of the heat exchange vessel with the liquid reservoir tanks;
a pump disposed on the tubular flow channel, and configured to circulate the liquid between the heat exchange vessel and the liquid reservoir tank; and
a switching valve disposed on the tubular flow channel, and configured to control the flow of the circulating liquid, which controls the temperature of the reaction vessel to keep a desired temperature by switching the flows of the liquids of the predetermined temperatures from the plurality of liquid reservoir tanks into the heat exchange vessel at a predetermined time interval,
wherein the amount of the sample is less than or equal to several µL per well, and the total volume of the circulating liquid is more than or equal to several tens of mL per liquid reservoir tank.</p> |
