SCREENING CELLS FOR A PHENOTYPE USING SUBPOPULATIONS HAVING SIMILAR CELL DENSITIES BY GROWING IN LIMITING NUTRIENT MEDIUM

摘要

Described herein are methods for obtaining from an initial population of cells a plurality of subpopulations of cells having a similar density by growing samples from the initial population in medium having a limiting nutrient. Also provided are methods for using such subpopulation of cells to identify members of an initial population, either spontaneously occurring or induced by mutagenesis, having altered and/or preferred phenotypes.