| 主权项 |
1. A prognostic method for determining the severity of disease in a patient diagnosed with or suspected of having breast cancer comprising:
a) obtaining a cell or tissue sample from a human subject diagnosed with or suspected of having breast cancer; b) contacting the sample with an antibody that binds a PCBP-1 antigen, wherein the antibody comprises a heavy chain variable domain comprising three complementarity determining regions (CDRs) comprising the amino acid sequences of SEQ ID NO: 45, SEQ ID NO: 46, and SEQ ID NO: 47, and a light chain variable domain comprising three CDRs comprising the amino acid sequences of SEQ ID NO: 48, SEQ ID NO: 49, and SEQ ID NO: 50; c) detecting the antibody; d) scoring the detection pathologically, wherein a pathology score of 0 indicates no staining in the cell, a score of 1+ indicates weak nuclear staining in any number of cells, or cytoplasmic staining in less than 30% of cells; a score of 2+ indicates weak cytoplasmic staining in 50% or more cells or strong cytoplasmic staining in more than 30% of cells; and a score of 3+ indicates strong cytoplasmic staining in more than 50% of cells; and e) providing a prognosis to the patient, wherein the pathology score correlates with the severity of disease, wherein a score of 0 or 1+ indicates a higher likelihood of patient survival and a score of 2+ or 3+ indicates a lower likelihood of patient survival. |
