IMPROVED RACE

摘要

The present invention relates to a method of generating a cDNA molecule or pool of cDNA molecules which comprise adapter sequences 5' and 3' of the sequence corresponding to the 5' or 3' ends of the original mRNA molecule. There is further provided a method of amplifying a desired 5' or 3' end of said modified cDNA or pool of cDNA molecules whilst minimising the generation of false positives and/or non-specific amplification. There is also provided a kit for carrying out the method of the present invention.