| 摘要 |
The invention relates to a method for producing polyunsaturated fatty acids in seeds of transgenic plants. According to said method, nucleic acids, coding for polypeptides with aω-3-desaturase,Δ-12-desaturase,Δ-6-desaturase,Δ-6-elongase,Δ-5-desaturase,Δ-5-elongase and/orΔ-4-desaturase activity, preferably for polypeptides with aΔ-6-desaturase,Δ-6-elongase andΔ-5-desaturase activity, are introduced into the organism. The nucleic acid sequences are represented by SEQ ID NO: 11, SEQ ID NO: 27, SEQ ID NO: 193, SEQ ID NO: 197, SEQ ID NO: 199 and SEQ ID NO: 201. Advantageously, said nucleic acid sequences can be expressed in the organism optionally together with other nucleic acid sequences that code for polypeptides of the biosynthesis of the fatty acid or lipid metabolism. Nucleic acid sequences coding for aΔ-6-desaturase,Δ-5-desaturase,Δ-4-desaturase,Δ-1 2-desaturase and/orΔ-6-elongase activity are especially advantageous. Advantageously, said desaturases and elongases originate from thalassiosira, euglena or ostreococcus. The invention also relates to a method for producing oils and/or triacylglycerides with an increased content of long-chain polyunsaturated fatty acids. In a preferred embodiment, the invention also relates to a method for producing arachidonic acid, eicosapentaenic acid or docosahexaenic acid, and to a method for producing triglycerides with an increased content of unsaturated fatty acids, especially arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid, in transgenic plants, preferably in seeds of the transgenic plants. The invention further relates to the production of a transgenic plant with an increased content of polyunsaturated fatty acids, especially arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid, based on the expression of the elongases and desaturases used in the inventive method. The invention also relates to recombinant nucleic acids molecules containing, together or individually, nucleic acid sequences coding for the polypeptides with aΔ-6-desaturase,Δ-6-elongase,Δ-5-desaturase andΔ-5-elongase activity, and transgenic plants containing said recombinant nucleic acid molecules. Another part of the invention relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use thereof. Furthermore, the invention relates to unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids, and to the use of the same. |
