发明名称 |
BOVINE IMMUNODEFICIENCY VIRUS (BIV) BASED VECTORS |
摘要 |
This invention pertains to BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter operably linked to the DNA segment; and a transgene operably linked to a second promoter; b) a BIV packaging vector construct comprising a BIV DNA sequence fragment comprising at least a gag gene or pol gene of BIV; a promoter operably linked to the BIV DNA fragment; and a polyadenylation sequence located downstream of the BIV DNA fragment; and c) an expression vector construct comprising a gene encoding a viral surface protein. Also provided is a method for transferring a gene of interest into a mammalian cell.
|
申请公布号 |
CA2392221(C) |
申请公布日期 |
2012.01.31 |
申请号 |
CA20002392221 |
申请日期 |
2000.12.13 |
申请人 |
NOVARTIS AG |
发明人 |
LUO, TIANCI;BERAHOVICH, ROBERT DAVID;KALEKO, MICHAEL |
分类号 |
C12N15/09;C12N15/86;A61K35/12;A61K35/76;A61K38/00;A61K48/00;C12N5/10;C12N7/00;C12N15/00;C12N15/867;C12N15/87;C12R1/91;C12R1/93 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|