| 摘要 |
In order to obtain a temperature gradient elution method which is rapid and accurate, a mobile phase (1) is supplied through two mobile-phase flow channels (2a) and (2b), mixed together, and introduced into the column (15) while the column (15) is made adiabatic. In this operation, the temperature of the mobile phase in one mobile-phase flow channel, i.e., the channel (2a), is regulated to a constant temperature higher than the upper limit of the target temperature range to be obtained in the column (15), while the temperature of the mobile phase in the other mobile-phase flow channel, i.e., the channel (2b), is regulated to a constant temperature lower than the lower limit of the target temperature range in the column (15). By controlling the flow rates in the two mobile-phase flow channels (2a) and (2b), the proportion in which these mobile-phase portions are mixed is changed with time to thereby change the temperature of the mobile phase in the column (15) with time. The temperature gradient elution method is thus conducted. |
