发明名称 |
Assay methods using chemiluminescent detection of peroxidase |
摘要 |
Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability. |
申请公布号 |
US8106203(B2) |
申请公布日期 |
2012.01.31 |
申请号 |
US20090476668 |
申请日期 |
2009.06.02 |
申请人 |
AKHAVAN-TAFTI HASHEM;DE SILVA RENUKA;XIE WENHUA;LUMIGEN, INC. |
发明人 |
AKHAVAN-TAFTI HASHEM;DE SILVA RENUKA;XIE WENHUA |
分类号 |
C07D219/00;C07D219/02;C07D219/04;C07D219/06;C07D409/04;C07D409/06;C07D411/04;C07D411/06;C07F9/64;C07F9/6558;C12Q1/28;G01N33/58 |
主分类号 |
C07D219/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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