METHOD OF LOADING AND DISTRIBUTING CELLS IN A BIOREACTOR OF A CELL EXPANSION SYSTEM

摘要

A method of distributing a plurality of cells in a bioreactor of a cell expansion system includes manipulating an orientation of the bioreactor such that a net impulse due to gravity acting on the plurality cells in the bioreactor is reduced. One method includes (a) rotating the bioreactor at an angular velocity ? about an axis of rotation and through an angular displacement ?, the bioreactor rotating from a first orientation to a second orientation; (b) holding the bioreactor still at the second orientation for a first period of time t1, wherein t1 substantially equals 2/?; (c) rotating the bioreactor at the angular velocity ? about the axis of rotation and through the angular displacement ?; and (d) holding the bioreactor still for a second period of time t2, wherein t2 substantially equals t1.