发明名称 |
Efficient Method For Loading Amphoteric Liposomes With Nucleic Acid Active Substances |
摘要 |
A method for preparing amphoteric liposomes loaded with polyanionic active agent as cargo, characterised by admixing an aqueous solution of said polyanionic active agent and an alcoholic solution of one or more amphiphiles and buffering said admixture to an acidic pH, said one or more amphiphiles being susceptible of forming amphoteric liposomes at said acidic pH, thereby to form such amphoteric liposomes in suspension encapsulating said active agent under conditions such that said liposomes form aggregates, and thereafter treating said suspension to dissociate said aggregates. Also disclosed are nucleic acid loaded amphoteric liposomes produced in accordance with the method, wherein said nucleic acids are oligonucleotides and said liposomes are multilamellar. |
申请公布号 |
US2012021042(A1) |
申请公布日期 |
2012.01.26 |
申请号 |
US20070225030 |
申请日期 |
2007.03.16 |
申请人 |
PANZNER STEFFEN;ENDERT GEROLD;RAUCHHAUS UNA;HERZOG NATALIE;MULLER CLAUDIA |
发明人 |
PANZNER STEFFEN;ENDERT GEROLD;RAUCHHAUS UNA;HERZOG NATALIE;MULLER CLAUDIA |
分类号 |
A61K9/127;A61K31/713;A61K38/02;A61K48/00;C12N5/00 |
主分类号 |
A61K9/127 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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