| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a process for amplifying a target nucleic acid sequence, which employs compartmentalization such that each compartment contains a single or at most 2-3 target nucleic acid molecules. <P>SOLUTION: There is provided a process for clonal pre-amplification of a nucleic acid, comprising (a) a step of providing a plurality of different nucleic acid molecules, (b) a step of attaching adaptor sequences to the 3' ends and 5' ends of the nucleic acid molecules, (c) a step of preparing a water in oil emulsion characterized in that the majority of water droplets comprises one or none member of the plurality of different nucleic acid molecules, and (d) a step of clonally amplifying the plurality of different nucleic acid molecules. In particular, the different nucleic acid molecules are mRNA molecules. <P>COPYRIGHT: (C)2012,JPO&INPIT |
