发明名称 |
2'-TERMINATOR RELATED PYROPHOSPHOROLYSIS ACTIVATED POLYMERIZATION |
摘要 |
<p>The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2′-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2′-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.</p> |
申请公布号 |
EP2076606(B1) |
申请公布日期 |
2012.01.25 |
申请号 |
EP20070819065 |
申请日期 |
2007.10.17 |
申请人 |
ROCHE DIAGNOSTICS GMBH;F. HOFFMANN-LA ROCHE AG |
发明人 |
GELFAND, DAVID HARROW;BAUER, KEITH A.;GUPTA, AMAR P.;BODEPUDI, VEERAIAH;NIEMIEC, JOHN |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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