| 摘要 |
The invention is a method for detecting a change in the conformational or energetic state of a polynucleotide processive enzyme during or after a reaction, comprising the steps of:
(i) immobilising the enzyme, under conditions suitable for the reaction to occur;
(ii) contacting at least part of the enzyme with a localised electromagnetic field; and
(iii) detecting a change in dielectric constant during or after the reaction, to thereby detect a change in the conformational or energetic state of the enzyme wherein the localised electromagnetic field is created by positioning the enzyme between first and second electrodes and applying an electrical signal to the first electrode. |
