发明名称 |
Compositions and methods for detecting phosphomonoester |
摘要 |
The invention provides a method of modifying a phosphomonoester moiety of a target compound. The method can include the steps of (a) providing a target compound having an electrophilic moiety and a phosphomonoester moiety; (b) contacting the target compound with a first carbodiimide compound under conditions for preferential addition of the first carbodiimide compound to the electrophilic moiety over the phosphomonoester moiety, thereby forming an electrophile-protected target compound; and (c) contacting the electrophile-protected target compound with a second carbodiimide compound and a nucleophilic compound under conditions for addition of the nucleophilic compound to the phosphomonoester. |
申请公布号 |
US8101375(B2) |
申请公布日期 |
2012.01.24 |
申请号 |
US20100868524 |
申请日期 |
2010.08.25 |
申请人 |
KOZLOV IGOR;MELNYK PETER;ZHAO CHANFENG;ILLUMINA, INC. |
发明人 |
KOZLOV IGOR;MELNYK PETER;ZHAO CHANFENG |
分类号 |
C12Q1/48 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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