| 摘要 |
FIELD: medicine, pharmaceutics. ^ SUBSTANCE: invention relates to medicine, namely to pharmaceutics. Elaborated is chemically standardised synthetic three-component complex Invaplex, including invasive proteins IpaB and IpaC and lipolysaccharide from Gram-negative bacteria. Sertotype-specific lipopolysaccharide component can include two or more lipopolysaccharides, selected from, at least, two different serotypes of Gram-negative bacteria. Elaborated is method of obtaining complex, which includes combination of IpaB and IpaC, with, at least, one lipopolysaccharide, bound with serotype of Gram-negative bacteria, stage A) being carried out as two separate mixing stages, including mixing of IpaB and IpaC with formation of complex IpaBTpaC and mixing complex IpaB:IpaC with, at least, one lipopolysaccharide (LPS) with formation of synthetic Invaplex and its isolation. ^ EFFECT: elaborated is chemically standardised synthetic three-component complex Invaplex for immunisation of subject, transportation of molecule through cell membrane, possessing improved properties due to stability of composition and biological activity. ^ 34 cl, 2 ex, 8 tbl, 13 dwg |
