Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy

摘要

P:/OPER/AJH/Specs/30842743 - Div Filing.doc-10/09/2009 Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then 5 genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.