The invention provides a method for increasing the stability and/or activity of a polypeptide at low pH and/or elevated temperatures. The invention further provides a method for increasing the melting termperature of a polypeptide. Also provided are paleoenzymologically reconstructed thioredoxin polypeptides having activity at higher temperatures and/or lower pH than extant thioredoxin polypepetides, as well as paleoenzymologically reconstructed thioredoxin polypeptides having higher melting temperatures than extant thioredoxin polypepetides.
申请公布号
WO2012009693(A1)
申请公布日期
2012.01.19
申请号
WO2011US44275
申请日期
2011.07.15
申请人
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK;GEORGIA TECH RESEARCH CORPORATION;FERNANDEZ, JULIO, M.;PEREZ-JIMENEZ, RAUL;GAUCHER, ERIC;KOSURI, PALLAV