发明名称 |
METHOD OF DETECTING AND QUANTIFYING WHEAT ENDOGENOUS DNA SEQUENCE |
摘要 |
<p>It is an object of the present invention to provide partial sequences of endogenous wheat DNA (genome) which are single copies and which allow wheat to be specifically detected without cross-reacting with other plants in PCR, and to provide primers for amplifying these partial sequences, along with a good method for detecting and quantifying endogenous DNA using these primers. The present invention provides, in a method for detecting or quantifying an endogenous wheat DNA sequence in a test sample by means of a polymerase chain reaction, a method comprising a step of using a nucleic acid in the test sample or a nucleic acid extracted from the test sample as a template to amplify the nucleic acid of a region comprising at least 80% or more of a nucleotide sequence represented by one of SEQ ID NO:1 through SEQ ID NO:7 using a primer pair capable of amplifying that region, and a step of detecting or quantifying the amplified nucleic acid.</p> |
申请公布号 |
KR20120005057(A) |
申请公布日期 |
2012.01.13 |
申请号 |
KR20117031339 |
申请日期 |
2005.04.06 |
申请人 |
NISSHIN SEIFUN GROUP INC.;INCORPORATED ADMINISTRATIVE AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION |
发明人 |
HINO AKIHIRO;KODAMA TAKASHI;IIDA MAYU;YAMAKAWA HIROHITO;NOZAKI SATOMI;HAYAKAWA KATSUYUKI |
分类号 |
C12Q1/68;C12N15/09;C12N15/11;G01N33/53;G01N33/566 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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地址 |
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