| 摘要 |
The present invention concerns a microorganism genetically modified for the bioproduction of 1,3-propanediol from sucrose,wherein the microorganism comprises: -a two-step metabolic pathway for the production of 1,3-propanediol, comprising a first step of decarboxylation of4-hydroxy-2-ketobutyrate with an enzyme having a 2-keto acid decarboxylase activity, and a second step of reduction of the obtained 3-hydroxypropionaldehyde with an enzyme having hydroxy aldehyde reductase activity,and -genes enabling the microorganism to utilize sucrose as sole carbon source. The invention also concerns a new method for the biological preparation of 1,3- propanediol by fermentation,comprising cultivating said microorganism genetically modified, wherein the culture is performed in an appropriate medium comprising a source of sucrose, and recovering the 1,3-propanediol being produced. In a preferred aspect of the invention, the source of sucrose is obtained from plant biomass. |
