A "tandem" reporter construct is disclosed that is capable of assaying RNA transcription termination. The ratio of expression between an upstream reporter and a downstream reporter as compared to the ratio observed for a control construct provides a measure of the relative rate of successful elongation through the intervening sequence. In one embodiment, two self-cleaving ribozymes separate the reporters from a test sequence between them.
申请公布号
WO2012005982(A2)
申请公布日期
2012.01.12
申请号
WO2011US41815
申请日期
2011.06.24
申请人
BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE;GRABCZYK, ED;SAMMARCO, MIMI, C.