| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a rapid, specific and sensitive immunochromatographic (ICT) assay for the detection of Streptococcus pneumoniae bacteria. <P>SOLUTION: A C-polysaccharide cell wall antigen is obtained from Streptococcus pneumoniae bacteria, thereby obtaining an antigen-specific antibody against the Streptococcus pneumoniae bacteria by using the antigen. Moreover, the ICT assay device is manufactured by using the antigen. A portion of such antibodies is conjugated to a labeling substance which displays a visible color change and embedded in a first zone of an immunochromatographic assay device. Another portion of such antibodies is bound to the reaction zone of the device which has a view window. When a liquid sample, such as patient urine, cerebrospinal fluid or blood is applied to the first zone, the conjugate of antibodies and labeling agent and the sample move along a flow strip of bibulous material to the reaction zone wherein, if the sample contains Streptococcus pneumoniae bacteria or its cell wall antigen, a sandwich is formed among the labeled conjugate, the antigen and the bound antibodies and a color change is observed. This assay affords a basis for rapid and reliable diagnosis of various pathogenic states caused by Streptococcus pneumoniae bacteria. <P>COPYRIGHT: (C)2012,JPO&INPIT |
