发明名称 |
SOLID GEL AMPLIFICATION METHOD AND APPARATUS FOR GENOTYPING AND PATHOGEN DETECTION |
摘要 |
Methods for detecting nucleic acid molecules comprising the use of polyacrylamide gel and PGR reagents photopohmerized together in a mold to create an array of semisolid posts that serve as reaction vessels for parallel, small volume PGR amplification of template DNA. Addition of a fluorescent marker prior to photopohmerization allows for the acquisition of real-time PGR data and melting curve analysis directlv from the mold. |
申请公布号 |
WO2012003579(A1) |
申请公布日期 |
2012.01.12 |
申请号 |
WO2011CA00789 |
申请日期 |
2011.07.08 |
申请人 |
THE GOVERNORS OF THE UNIVERSITY OF ALBERTA;ATRAZHEV, ALEXEY;ACKER, JASON |
发明人 |
ATRAZHEV, ALEXEY;ACKER, JASON |
分类号 |
C12Q1/68;C12M1/40;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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