IMPROVED DETECTION SIGNAL AND CAPTURE IN DIPSTICK ASSAY

摘要

PROBLEM TO BE SOLVED: To provide an improved dipstick assay for testing on the presence of a target nucleic acid in a sample solution.

SOLUTION: A dipstick is provided which comprises a contact end for contacting the sample solution and a capture zone remote from the contact end for capturing the target nucleic acid. The sample solution is contacted with the contact end to be moved to the capture zone by capillary action. The target nucleic acid in the sample solution is captured at the capture zone and is detected by a plurality of different labeled detection probes, each of which is capable of hybridizing to a different region of the target nucleic acid. The detection signal is thereby enhanced. In another method, a plurality of different capture probes are added to the sample solution, then the capture probes are bound by a capture moiety at the capture zone and the target nucleic acid is captured indirectly. The capture of the target nucleic acid is thereby improved. Kits and dipsticks for performing such methods are also described.

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