The invention refers to a method of producing a recombinant polypeptide of interest (POI) in a cell culture, comprising genetically engineering a eukaryotic cell line - to specifically cause prolongation of the G2+M cell cycle phase in a pre-culture phase, and - to produce the POI in a producing phase following the pre-culture phase, a high producer cell line and cell culture as well as a method of increasing the yield of a recombinant POI production in a cell culture.
申请公布号
WO2012004226(A1)
申请公布日期
2012.01.12
申请号
WO2011EP61241
申请日期
2011.07.04
申请人
UNIVERSITAET FUER BODENKULTUR WIEN;MATTANOVICH, DIETHARD;DRAGOSITS, MARTIN;GASSER, BRIGITTE;MAURER, MICHAEL;SAUER, MICHAEL
发明人
MATTANOVICH, DIETHARD;DRAGOSITS, MARTIN;GASSER, BRIGITTE;MAURER, MICHAEL;SAUER, MICHAEL