METHOD AND KIT FOR DETECTING EGFR MUTATION BY USING PNA-BASED REAL-TIME PCR CLAMPING

摘要

The present invention relates to a method for selectively detecting only a mutant by inhibiting the amplification of wild type by using a peptide nucleic acid (PNA) probe which specifically binds to wild type of in frame deletion or substitution portions at exon 18, 19, 20 or 21 where an EGFR mutation gene exists, and a kit for using the method. It is possible to rapidly and accurately examine tumors of prostate cancer, breast cancer, colon cancer, pancreatic cancer, ovarian cancer, spleen cancer, testicular cancer, thymus cancer, lung cancer and the like in the early stages by using the present invention, thereby enabling effective treatment due to cancer diagnosis at an early stage.