DETECTION AND QUANTIFICATION OF VITAMIN D METABOLITES USING AN ALKYLAMINE TO FORM STABLE ADDUCTS FOR MASS SPECTROMETRIC ANALYSIS

摘要

The invention describes a method of determining the amount of an analyte in a sample wherein the analyte comprises one or more vitamin D metabolites; comprising the steps of; contacting the analyte with an alkylamine to form an alkylamine adduct; subjecting the adduct to a MS technique to determine the amount of the adduct; and calculating the amount of the analyte equivalent to the amount of the adduct.