发明名称 |
SNP-DEPENDENT END LABELING METHOD |
摘要 |
Certain embodiments described in this disclosure relate to a method of sample analysis. In certain cases, the method comprises: a) contacting a genomic sample comprising double-stranded genomic DNA with a first restriction endonuclease that recognizes a nucleotide sequence that comprises a SNP site in the double stranded genomic DNA, wherein: i. the restriction endonuclease cleaves the genomic DNA at the sequence regardless of the allele of the SNP present at the SNP site; and ii. cleavage of the sequence by the restriction enzyme creates a 5′ overhang that comprises the SNP site; b) contacting the digested genomic sample with a extension enzyme and a first labeled nucleotide that is used by the extension enzyme to fill in the overhang only if the overhang comprises a first allele of the SNP. |
申请公布号 |
US2012004123(A1) |
申请公布日期 |
2012.01.05 |
申请号 |
US20100829155 |
申请日期 |
2010.07.01 |
申请人 |
PETER BRIAN JON;SAMPAS NICHOLAS M. |
发明人 |
PETER BRIAN JON;SAMPAS NICHOLAS M. |
分类号 |
C40B30/04;C12Q1/68;C40B40/06 |
主分类号 |
C40B30/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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