| 摘要 |
<p>Determining a protease preferably when protease is present in a form after its proform and in a form that is inhibited by a protease inhibitor, in a biological sample, comprises: converting the proform of the protease into an active protease; contacting the test sample with a substrate, at which an inhibitor binding substance is bound; and separating the substrate bound with protease inhibitor from the test sample, where a substrate for the protease whose activity to be determined is added to the test sample and the proteolytic conversion of the substrate is determined by the protease. Determining a protease preferably when protease is present in a form after its proform and in a form that is inhibited by a protease inhibitor, in a biological sample, comprises: converting the proform of the protease into an active protease; contacting the test sample with a substrate, at which an inhibitor binding substance is bound covalently or by adsorption, where the inhibitor binding substance has higher affinity (binding strength) to the protease inhibitor than the protease; and separating the substrate bound with protease inhibitor from the test sample, where a substrate for the protease whose activity to be determined is added to the test sample and the proteolytic conversion of the substrate is determined by the protease. An independent claim is included for a device for performing the above method, where the substrate is agarose gel or cellulose.</p> |
