METHOD FOR DETERMINATION OF ACTIVITY OF MITOCHONDRIAL DNA POLYMERASE OF PLASMODIUM FALCIPARUM MALARIA, AND METHOD FOR SCREENING FOR ANTI-MALARIA COMPOUND

摘要

Disclosed is a means which is useful for the development of an anti-malaria agent. It is found that a mitochondrial DNA polymerase of plasmodium falciparum malaria requires a bivalent iron ion. Thus, disclosed is a method for determining the activity of a DNA polymerase, comprising the steps of: (1) incubating a solution containing a bivalent iron ion, a mitochondrial DNA polymerase of plasmodium falciparum malaria, template DNA, and at least one deoxyribonucleoside triphosphate or deoxyribonucleoside triphosphate derivative to synthesize double-stranded DNA; (2) detecting the synthesized double-stranded DNA; and (3) calculating the activity of the DNA polymerase from the results of the detection carried out in step (2).