| 摘要 |
The purpose is to construct a closed system having a simple structure without resorting to large scale equipment, wherein a step for separating useful cells, a step for culturing the useful cells separated in the separation step and a step for washing and concentrating the cells cultured in the culture step are all consistently carried out, to thereby enable the preparation of useful cells having high safety and good qualities while improving the handling properties. A cell culture device comprising a disposable set for cell culture, channel-switching valves and pumps that are provided at appropriate positions in the pipeline of the disposable set and a control unit for controlling the same, wherein said disposable set comprises: a cell culture container (CC) provided with a liquid inlet (Lin) and a liquid outlet (Lout); a cell separation kit (A) for separating cells to be used in the cell culture, said separation kit being connected to the liquid inlet (Lin); and a cell collection kit (B) for washing and concentrating the cells cultured in the cell culture container (CC), said cell collection kit being connected to the liquid outlet (Lout). |
