Method for increasing the expression of a recombinant protein and plasmid yield in an eukaryotic or prokaryotic high cell density culture

摘要

The present invention relates to a method for increasing the expression of a recombinant protein and plasmid yield in an eukaryotic or prokaryotic high cell density culture. The increase of the expression is achieved by the use of perfluorochemicals as ingredients of an enzyme-based fed-batch cultivation system by enhancing the cell growth rate and the final cell density.