METHOD AND KIT FOR IDENTIFICATION OF MYCOBACTERIUM SP. USING REAL-TIME PCR

摘要

PURPOSE: A method for simply and accurately detecting mycobacterium species by multi probe PCR is provided. CONSTITUTION: A method for detecting mycobacterium species comprises: a step of adding a probe of sequence numbers 4-13 or sequence number to perform real-time PCR; and a step of reading mycobacterium species by confirming Tm value of a PCR product. The Mycobacterium includes M. abscessus, M.avium, M.fortuitum, M.intracellulare, M.kansasii, M.massiliense or M.tuberculosis. A kit for detecting mycobacterium contains a primer of sequence numbers 1-3 or a probe of sequence numbers 4-13.