METHODS FOR PRODUCING SOLUBLE MULTI-MEMBRANE-SPANNING PROTEINS

摘要

METHODS FOR PRODUCING SOLUBLE MULTI-MEMBRANE-SPANNING PROTEINS AbstractMethods for producing membrane-spanning polypeptides in high yields, with nativeconformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts. No suitable figure