发明名称 |
EVALUATION PEPTIDE FOR USE IN QUANTIFICATION OF PROTEIN USING MASS SPECTROMETER, ARTIFICIAL STANDARD PROTEIN, AND METHOD FOR QUANTIFYING PROTEIN |
摘要 |
Disclosed is an evaluation peptide for evaluating the efficiency of a pretreatment in the quantification of a protein using a mass spectrometer, having high reliability and high general versatility. Also disclosed is an artificial standard protein comprising the evaluation peptide. Further disclosed is a method for quantifying a protein utilizing the artificial standard protein. Specifically disclosed is a method for selecting a peptide which consists of an amino acid sequence not agreeing with that in a naturally occurring protein and a variant thereof and capable of being detected by mass spectrometry and which has an amino acid that can be recognized by a protein-digesting enzyme, and using the peptide as an evaluation peptide for use in the quantification of a protein by a mass spectrometer. |
申请公布号 |
EP2400295(A1) |
申请公布日期 |
2011.12.28 |
申请号 |
EP20100743497 |
申请日期 |
2010.01.27 |
申请人 |
TOHOKU UNIVERSITY |
发明人 |
OHTSUKI, SUMIO;KAMIIE, JUNICHI;TERASAKI, TETSUYA |
分类号 |
G01N27/62;C07K5/08;C07K5/10;C07K7/00;C07K7/06;C07K7/08;G01N33/68;H01J49/00 |
主分类号 |
G01N27/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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