Gene expression technique

摘要

The invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising:
(a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of Orm2p or a variant or fragment thereof and a second gene, such as a second recombinant gene, encoding a desired protein (such as a desired heterologous protein), with the proviso that the first and second genes are not present within the host cell on the same 2µm-family plasmid; and
(b) culturing the host cell in a culture medium to obtain expression of the first and second genes.
The invention also provides related uses, plasmids and host cells.