发明名称 |
METHOD FOR GENE AMPLIFICATION |
摘要 |
The present invention provides a double-stranded DNA constructed specifically for high speed gene amplification, a method for gene amplification and a method for synthesizing protein. The gene amplification system of the present invention used a site-specific recombinase such as Cre-lox system and target sequence thereof to efficiently induce a type of replication referred to as a double rolling-circle replication (DRCR). Amplification unit, whose structure is shown in Figure 2 (a) , is constructed in animal and other cells. DRCR is induced by two recombination events triggered by a site-specific recombinase (Cre) when each replication folk progresses between each pair of target sequences (lox sequences). |
申请公布号 |
EP1967585(B1) |
申请公布日期 |
2011.12.28 |
申请号 |
EP20060768267 |
申请日期 |
2006.07.18 |
申请人 |
INTER-UNIVERSITY RESEARCH INSTITUTE CORPORATION NATIONAL INSTITUTES OF NATURAL SCIENCES |
发明人 |
HORIUCHI, TAKASHI;WATANABE, TAKAAKI |
分类号 |
C12Q1/68;C12N15/10;C12N15/69;C12N15/90;C12P21/02 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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