发明名称 |
Method and apparatus for single side bilayer formation |
摘要 |
An apparatus for single-sided bilayer formation includes a first fluid chamber including a sidewall and a second fluid chamber extending through the sidewall. A barrier wall separates the first and second fluid chambers and includes a nanopore therein across which a planar lipid bilayer (PLB) is formed. In use, an electrolyte is added to the first and second fluid chambers and a lipid/organic solvent mixture is added to the first fluid chamber to form a lipid/organic solvent layer. The electrolyte level within the first fluid chamber is adjusted such that the lipid layer is raised above the barrier wall and a PLB is formed. Electrolyte levels may be adjusted manually or utilizing a fluid level regulator with or without feedback control. Optionally, the apparatus may be in the form of a nanopore array. The apparatus may be incorporated into an ion channel sensing system wherein an electrical current through each PLB is separately recorded. |
申请公布号 |
GB2467479(B) |
申请公布日期 |
2011.12.28 |
申请号 |
GB20100007820 |
申请日期 |
2008.12.01 |
申请人 |
ELECTRONIC BIO SCIENCES LLC |
发明人 |
ERIC N ERVIN;GEOFFREY ALDEN BARRALL |
分类号 |
G01N27/30;H01B1/12;H01M2/36 |
主分类号 |
G01N27/30 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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